Confocal of Late Pupal Butterfly Wing
by Steve Paddock

Special Scientific Events
Pre-meeting Congress: August 4 - August 5
Full Days Starting at 8:00AM

Imaging Life: From Cells to Whole Animals
Organizer: David Piston

A convergence of newly developed instrumentation and optical probes is allowing researchers to examine dynamic processes within ever more complicated biological systems. These new tools are affecting how almost all biological optical microscopy experiments are done. Studies of single cells, for instance, have dramatically changed since the introduction of the Green Fluorescent Protein (GFP). Improvements in light sources and detectors also allow quantitative spectroscopic methods to be used in an imaging modality. These include fluorescence lifetime imaging, Raman microscopy, fluorescence correlation spectroscopy, resonance energy transfer microscopy, and single molecule techniques. In addition, non-fluorescent contrast methods such as polarization and Nomarski DIC have also benefited from technical improvements. As molecular imaging methods have been perfected in single cells, they have also been rapidly applied to imaging of tissues and whole organisms. Methods such as multi-photon excitation and luminescence imaging now offer an alternative to CT, MRI, and Ultrasound for many non-invasive in vivo imaging applications. This pre-meeting congress will bring together experts in both instrumentation and probe development in a broad discussion of the field and future directions. In addition, speakers will describe latest developments in live cell imaging from isolated cells, intact tissue, and whole organisms. The congress will be both timely and exciting to a wide range of researchers.

Saturday, August 4
7:00
Registration and Continental Breakfast
8:00Dave PistonIntroduction
8:15Ted SalmonUniversity North Carolina Fluorescence Speckle Microscopy
8:45Linda PieriniWeill Medical College of Cornell University Endocytic Recycling in Motile Neutrophils
9:15Rich DayUniversity Virginia Multi-spectral Imaging of GFP in Living Cells
9:45
Coffee
10:15Jason SwedlowUniversity of Dundee Chromatin Dynamics
10:45Ron LynchUniversity of Arizona Fluorescence Spectral Imaging Approaches for Physiological Analysis
11:15 Ken SpringNIH Methods for Imaging Living Epithelia
11:45
Lunch
1:00Tobias MeyerStanford University Use of GFP to Investigate Signal Transduction Pathways
1:30Wolf AlmersVollum Institute Imaging Secretion by Evanescent Field Microscopy
2:00Ed LevitanUniversity of Pittsburgh Imaging Neuropeptide Release with GFP
2:30Roger TsienUniversity of California, San Diego Use of GFPs for FRET Imaging
3:00
Coffee
3:30Brian HermanUniversity of Texas, San Antonio FRET Determination of Protein-Protein Interactions in Living Cells
4:00Phillip BastiaensEMBL Heidelberg Use of Fluorescence Lifetime Imaging for FRET Analysis
4:30Peter VanderklishScripps Research Institute Use of FRET Microscopy to Study Neuronal Function
5:00Mark RizzoVanderbilt University FRET Imaging of Glucose Sensing in Pancreatic b Cells
5:30
Poster Session/Exhibits/Snacks/Drink
Sunday, August 5
7:00
Continental Breakfast
8:00Paul RobinsonPurdue University Three Dimensional Imaging of Matrices for Artificial Organ Development
8:30David MillerVanderbilt University Color Coding the C. elegans Nervous System
9:00Bill MohlerUniversity of Connecticut C. elegans Development
9:30Jayne SquirrellUniversity of Wisconsin Non-linear Microscopic Imaging of Development
10:00
Coffee
10:30Stefan HellMax-Planck-Institute for Biophysical Chemistry Multi-focal Multi-photon Microscopy of Living Cells
11:00 Mary DickinsonCalTech Spectral Imaging with Multiphoton Excitation Microscopy
11:30Peter SoMassachusetts Institute of Technology In Vivo Two-Photon Characterization of Tissue Physiologyy
12:00
Lunch
1:15Simon WatkinsUniversity of Pittsburgh Hand-held Laser Scanning Microscope for In Vivo Imaging
1:45Rakesh JainHarvard/MGH In Vivo Imaging of Gene Expression, Physiological Function and Therapeutic Response in Tumors
2:15David KlienfeldUniversity of California, San Diego Imaging Neural Function in Living Mice
2:45
Coffee
3:15Chris ContagStanford University In Vivo Luciferase Imaging
3:45Frank CarrollVanderbilt University Monochromatic X-rays for In Vivo Imaging
4:15Simon CherryUniversity of California, Davis Imaging Whole Animals with MicroPET
4:45
Summary and Discussion


Nestor J. Zaluzec / Zaluzec@MSA.Microscopy.Com