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Organizers: August 4 |
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Course 02-01: Introduction to X-ray Energy Dispersive Spectrometry and Quantitative Analysis Instructor: Paul Carpenter |
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This course will cover practical applications of the energy-dispersive spectrometer (EDS) to X-ray microanalysis. Topics covered will include detector technology, advances in pulse processing, resolution and performance monitoring, detector modeling, peak deconvolution and fitting, qualitative and quantitative analysis, compositional mapping, and standards. An emphasis will be placed on use of the EDS for quantitative analysis, with discussion of typical problems encountered in the analysis of a wide range of materials and sample geometries. |
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Course 02-02: Digital Imaging 2002 Instructor: John Mackenzie, Jr. |
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This course will discuss the various strategies needed for producing digital data that is suitable for publication. Because this field is evolving so rapidly, the course material will change sufficiently that individuals may find it beneficial to repeat this course. We will discuss how to acquire the best digital image for a given sample. Noise removal and resolution issues will be discussed in detail. We will examine what the current best technologies for archiving the image data are and what image formats and standards we should adopt. We will examine in detail image printing. We will emphasize several issues that must be understood in order to produce high quality images every time on any printer (the most critical being the gamma correction). There will be a strong emphasis placed on the most affordable solutions available regardless of platform or operating system. We will examine the latest technologies such as digital cameras and digital video to see how they may best be applied to microscopy. We will discuss the major issues that must be addressed when moving to a more digital approach. |
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Course 02-03: Focused Ion Beam (FIB) Microscopy and Technology Instructors: Phil Russell and Fred Stevie |
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This course begins with a discussion of the basic concepts of using ions (rather than electrons or photons) as a basis for microscopy. The development of the very high brightness gallium liquid metal ion source (LMIS) and associated ion optics have resulted in FIB microscope/work- stations playing several major roles in microscopy and microanalysis labs. FIB optics with 5 nm resolution performance will be described and compared with SEM. Then, the unique features of FIB due to the ion mass and sputtering possibilities will be described. Contrast mechanisms when imaging with emitted electrons in a scanning ion beam system will be described with numerous examples. The interaction of ions with matter is presented to the extent needed to understand the sputtering process, and beam induced chemistry. The use of FIB as a micromachining system for numerous applications will be described. These include cross sectioning of bulk samples and in situ imaging; TEM sample preparation, and the fabrication of probes for scanned probe microscopy (STM and AFM). Examples of site specific TEM sample prep, including lift out methods, which allow for very low damage, atomic resolution specimen prep with rapid turn around time will be given. Ion beam induced chemistry techniques will be described which allow both material deposition with high spatial resolution and, with different chemistries, enhanced machining or material etch rates. The course will conclude with a discussion of current FIB instrumentation including system with FE-SEM columns incorporated into a dual beam configuration. |
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Course 02-04: Live Cell Imaging: A Primer Instructors: Simon Watkins and Nancy Burke |
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In the post-genomic era of biomedical research, understanding the functionality of molecules at the cellular and sub-cellular level in living systems will become predominant. In this area we must move beyond static ÒsnapshotsÓ of the cellular state to an understanding of the biology of cells over time and in 3-dimensional space. Within the cellular environment it is expected that we will be able to study the expression, the functional role(s) and interactions of multiple unique molecules concurrently. Furthermore, it will be desirable to determine the effects of these molecules on cell development, organization and fate over extended periods of time. To perform these types of studies it is necessary to develop new methodologies that will allow multi-parametric analysis of cells while maintaining their functional viability. In the past this goal would have been extraordinarily difficult to achieve. However, developments in optical and computational technology have empowered modern microscopists to undertake these previously forbidding tasks. This day long workshop will discuss live cell imaging tools, the expectations of the technology and limitations of optical tools within the context of current scientific efforts principally focusing on the use of fluorescent proteins and ratiometric tools in live cell methodologies. Lectures will include: optical principles, fluorescence principles, microscope design, cameras, Image archiving and management and multidimensional image analysis tools for live cell imaging. |
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Course 02-05: What the Heck Happened to This? Real Life Failure Analysis Tips and Tricks Using Microscopy and Microanalysis Instructor: Valerie Woodward |
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Most industrial service lab microscopists are faced with solving manufacturing and applied R&D problems on the fly with broad-use, commercially available, and sometimes outdated equipment using routine analytical methods and of course, yielding data yesterday and for $19.99! Although the questions about why materials fail (or work!) aren't always answered at the most fundamental levels, we do need to provide the best reasonable answers in the shortest reasonable times to our customers so that they can relate the problem to a specific process or material. Many times, we just need to give our customers a direction in which to proceed, and can follow up with the pesky details later; with this in mind, there are many alternate routes that we can take to give guidance. Of course, this obliges us to know about the materials and processes that we are examining, about maintaining a historical perspective on problems (i.e., being an old timer), and how to relate our information to the non-scientist as well as the technical customer. This talk will present some approaches to failure analysis, and a number of case studies that have required the use of multiple hierarchies of microscopy, microanalysis, microsampling, and multiple analytical techniques in order to provide timely and useful results to the customers. |
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Course 02-06: High pressure freezing plus freeze substitution equals a new approach to immunolabelling Instructor: Jan Slot |
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Introduction to High Pressure Freezing, HPF. Why HPF? Advantages of High Pressure Freezing over conventional Ð chemical and/or microwave fixation. After HPF what techniques/methods of tissue examination can be used Ð cryosectioning, cryoplaning, freeze substitution and/or immuno labeling. Techniques and examples of Immuno Labelled HPF tissues and cells For this course only (Course 02-06) you must call to register since it has been added after the registration booklet was printed. Contact Microscopy & Microanalysis 2002 Meeting Management at 877-MSA-MAS-1 and mention that you specifically need to register for the High Pressure Freezing Course. A maximum of 20 participants can register for this course. |