All Invited and/or Contributing Authors submitting papers for this meeting must select thier symposia from the list below . After your paper is prepared according
to the Author Instructions, you must complete the Author Data Form and then submit your
contribution Electronically and by Hard Copy. There are three type of
presentations at the meeting: Invited Platform Presentation , Contributed Platform Presentation, Contributed Poster Presentation. The instructions for preparation of papers for publication all three types of submissions are identical.
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Special Session
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Special Late Breaking Posters
Organizer: Edgar Voelkl
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The program committee frequently receives requests to exhibit timely and exciting new results that has been obtained past the February 15th deadline for the submission of papers for the meeting. This is your avenue for presenting late-breaking results at the meeting. If you would like to submit a "Late Breaking Poster", please contact the Program Chair: Edgar Voelkl at eVoelkl@nLine.com for further information. |
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Biological Sciences Symposia |
01. "3-D" Electron Microscopy of Macromolecules/Characterization of Macromolecules and their Function
Organizers: Peter Ottensmeyer and Joachim Frank
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The goal of molecular biology is to provide a comprehensive understanding of the functional and structural relationships of biological processes at the molecular level. While the two aspects have in general been studied in isolation, or been the purview of atomic level structural analysis in crystallography and NMR spectroscopy for a number of enzyme reactions, structural analysis by electron microscopy and image processing of single particles or of crystals have lately contributed greatly to our understanding of the function of major processes, such as those in protein synthesis and transmembrane signal transduction. The symposium and contributed papers will highlight electron microscopic structures in terms of their structure/function relationships at the level of macromolecules and their complexes in any biological field. |
02. Plant-microbes Interactions at the Cellular and Molecular Levels
Organizers: Pierre M. Charest and Suha Jabaji-Hare
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This session will present several aspects, research avenues and microscopic approaches for the study of the interactions between microorganisms including fungi and bacteria as well as virus with the plant cell. The contributed papers will cover topics related either to pathogenic microorganisms or to the beneficial soil microflora such as mycorrhizae and symbiotic bacteria. Topics dealing with real-time microscopy and confocal microscopy imaging will also be discussed as exciting powerful tools to study plant-microbe interactions at the cellular and molecular levels. Contributed papers and posters on related topics of plant pathogenic and symbiotic microorganisms are also welcome. |
03. The Microstructural Approach to Food Processing and Engineering
Organizers: Alejandro Marangoni and David Stanley
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Food structure is the organization of constituent macromolecular elements and their interaction. Food processing, a series of restructuring and reassembling operations, benefits from many newly developed techniques for examination and analysis of food microstructure. Examples of these techniques include deconvolution wide field microscopy, confocal laser scanning microscopy, environmental scanning electron microscopy, position tagged spectroscopy, scanning probe microscopy, and magnetic resonance imaging. Microstructural knowledge of foods remains critical in processing, packaging, storage, distribution and preparation because of the direct causal connection between structure and food product behaviors. This symposium will emphasize and encourage contributions that reflect microstructural approaches to food engineering and development, including both biological and materials evaluation and analysis. |
04. Advances and Applications in Vascular Corrosion Casting in Microvascular Research
Organizers: Fred Hossler and Seyedhossein Aharinejad
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While the traditional application of vascular corrosion casting has been to describe the three dimensional anatomy and distribution of blood vessels in tissues and organs, other applications have included monitoring changes in vasculature in disease and during normal development. Techniques for quantitation of the vasculature using corrosion casts have also become increasingly important. It is anticipated that this session will include new developments in each of these areas. |
05. Advances in Ultrastructural and Non-invasive Imaging of Skin
Organizer: Manoj Misra
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Recent advances in instrumentation and imaging techniques (in vivo confocal, Raman, OCT, 2-Photon, EPR imaging and cryo-EELS spectroscopic imaging, ESEM) have begun to offer new avenues to probe skin's chemical- and ultra-structure and thus develop improved understanding of skin physiology and function. This symposium aims to pull together such cutting-edge imaging techniques to address their potential and associated pitfalls in imaging skin. |
06. Electron Cryo-microscopy of Biological Macromolecules
Organizer: Wah Chiu
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The topics of the symposium will be the applications of electron cryomicroscopy and computer reconstruction to the study of biological assemblies at high resolution. Invited speakers will cover topics related to 2D crystal structure, helical arrays, and single particle analysis. Contributed platform and poster presentations using microscopy to study macromolecules are also encouraged. |
07. Confocal and Deconvolution for Biologists
Organizers: Elaine Humphrey and Jay Jerome
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This session will look at the way confocal and deconvolution microscopes have been used to image not only fixed material but also living systems. We will look at developments in imaging fluorochromes using conventional confocals, spinning disc confocals, and deconvolution systems and how the use of vital stains and fusion proteins such as GFP, have allowed an abundance of research into answering physiological questions such as how certain proteins interact within cells and tissues. We will explore the difficulties of keeping cells alive while collecting meaningful data. |
08. Advances in Linking Structure to Function in Biomaterials
Organizers: Christopher Siedlecki and Steven Eppell
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Biomaterials research is an interdisciplinary field requiring expertise in both physical and biological sciences. Traditional biomaterials provide the necessary mechanical and chemical properties for medical and drug delivery devices while eliciting minimal biological host response. Newer contributions include: biomaterials designed to elicit specific ÒappropriateÓ biological responses, self-assembling materials utilizing lessons from nature, and materials that can deliver drugs locally and at controlled rates. This symposium offers a platform for discussing recent advances in understanding the structure of biomaterials ranging from the nanometer (molecular) to the cm (tissue) scales. Contributions linking structural details with functional aspects of the materials (chemical, mechanical or biological) of both traditional and novel biomaterials are especially encouraged. |
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Physical Sciences Symposia |
09. In Situ Electron Microscopy Techniques and Applications/ Reactions
Organizers: Pratibha Gai and Eric Stach
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The study of in situ reactions is a rapidly advancing field in both materials and biological sciences. Dynamic studies by in situ microscopies provide direct insights into materials processes and properties, leading to exciting new developments in the materials and biological sciences, and in nanotechnology. This timely symposium will include high resolution environmental and high temperature electron microscopy under dynamic reaction conditions. In situ contributions of FESTEM, wet (solution) environmental microscopy, energy filtering and electron energy loss spectroscopy, FESEM, aberration correction, novel specimen stages and other related topics will be welcome. The symposium will cover applications and emerging opportunities in Catalysis, Surface and Interface reactions, the Structural Dynamics of Defects, Polymerization, Carbon and other Nanostructures, Crystal Growth, Deformation, Phase Transformations in Particulate Materials, Proteins on nanostructures and Electromigration. Symposium sessions will be dedicated to Prof. Sir P. B. Hirsch, a pioneer and leader of EM and MSA distinguished scientist. |
10. Polymer Characterization: It's Not Just For Microscopes Anymore
Organizers: Catherine Johnson and Valerie Woodward
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From optical observation of polyethylene spherulites to micro modulus measurements of polymer blends, polymer characterization has experienced much transition. Electron beams, X-rays and tunneling currents, to name a few, all play an important role in today's characterization studies. This symposium will cover Òtried and trueÓ through novel techniques used to explore the structure/property relationships of today's polymeric materials. Current industry demands increased versatility of material behavior in order to design polymers that are application specific. The desire to dial in properties requires one to understand materials from nanoscopic to macroscopic levels. The methods visited and revisited here will allow this desire to become reality in the very near future. |
11. Magnetic Materials and Super-conducting Materials
Organizer: Yi Liu
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Magnetic materials and super-conducting materials are among the key materials supporting next generation of compact and super-fast computers and other electronic devices. This symposium solicits presentations on progress in characterizing both the physical structures and magnetic structures of magnetic materials and super-conducting materials. Topics include but are not limited to scanning probe microscopy, magnetic force microscopy, atom probe microscopy, X-ray diffraction, neutron diffraction, Mossbauer spectroscopy, spin-polarized electron spectroscopy, scanning electron microscopy and transmission electron microscopy. We encourage microscopists and experts in magnetic materials and super-conducting materials to exchange information and team up for future collaboration. |
12. Advances in Nanoscale Technology
Organizers: Thomas Kelly, David Larson and Michael Thompson
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Pursuit of nanoscale technology is projected by many to be a major 21st century industrial activity. The microscopes needed to work on this scale will play a crucial role in enabling its successes. In many cases, the characterization tools become nanofabrication devices as well. This symposium will showcase the many techniques used for nanoscale characterization and fabrication of inorganic and organic structures and explore the role of microscopies in nanoscale technology. Metals, ceramics, semiconductors, polymers, and biological materials will all be considered. |
13. Microscopy and Microanalysis of Self-Organized Soft Condensed Matter
Organizers: Richard Spontak and Judith Yang
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Soft condensed matter composed of bi/multifunctional chemical species may, under the right conditions, self-organize into nanoscale or microscale structural elements that promote the development of unique properties or applications. Common examples of self-organizing species include amphiphiles, copolymers and polyelectrolytes. Real- and reciprocal-space characterization of such elements is crucial to an improved understanding of their development mechanism, stability and utility. Various microscopy methods, including, but not limited to, near- and far-field optical microscopy, scanning electron microscopy, transmission electron microscopy, X-ray microscopy, electron tomography and surface probe microscopy, have been employed to quantify the size, dispersity, shape, composition, symmetry, order and connectivity of structural elements formed upon the self-organization of soft condensed matter. This symposium will highlight advances and successes of microscopy methods used in this endeavor. |
14. Electron Microscopy of Macro-, Micro- and Meso-Porous Materials
Organizers: Manuel E. Brito and Douglas Blom
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In spite of the recent interest in the area of meso-porous materials and continued interest in the areas of macro- and micro- porous materials, characterization studies by electron microscopy have been rarely reported in the literature. The aim of our session is to develop effective interactions among electron microscopists, scientists, engineers and entrepreneurs in this field, in order to foster the spread of information. It will highlight creative sample preparation techniques, as well as the characterization of all aspects of porous materials. Characterization techniques will include SEM, conventional TEM, HRTEM, in-situ electron microscopy, Z-contrast imaging, cross-section STM and AFM, EDS, EELS, etc. Presentations dealing with the microstructure-property relationships of porous materials are encouraged. |
15. Quantitative X-ray Microanalysis in the Microprobe and in the SEM: Theory and Practice
Organizers: Raynald Gauvin and Eric Lifshin
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This symposium presents the latest developments in X-ray microanalysis for the electron microprobe, the SEM and ESEM. Because of the rapid progress in instrumentation, modeling techniques and software, X-ray microanalysis today is applicable in a broad range of experimental setups and a large diversity of samples. Topics of particular interest include: software relating to new correction and simulation procedures, microanalysis of light elements and spectra at low operating voltages and in FE-SEM's. Special consideration is given to X-ray analysis in the ESEM and VP-SEM, how to optimize spatial resolution and discussion of general procedures for quantitative analysis. Data from the X-ray microanalysis of rough surfaces, porous materials and multi-layer thin film structures, using improved measurements and computation of fundamental parameters describing X-ray emission, and electron diffusion will also be discussed. This symposium will demonstrate that new X-ray microanalysis methods continue to develop rapidly and provide a characterization tool that continues to meet the demand for increased capabilities. |
16. State of the Art Infrared and Raman Microanalysis
Organizers: Michael Jackson and Edgar Etz
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This symposium will highlight vibrational microanalysis in the materials, biological and analytical sciences. Topics will include: Fundamentals of inelastic scattering, Instrumental advances in synchrotron infrared microspectroscopy, confocal Raman microprobe/microscopy, Raman microspectroscopy and near-field IR and Raman microscopy. Vibrational spectroscopic imaging including instrument performance, biological/medical applications and analysis of data sets. Characterization of semiconductors, polymers, composites and ceramics. Vibrational microspectroscopy in biology, environmental studies, geological sciences, art and archaeology. Quantitation in Raman probe microspectroscopy, including instrument calibration and standardization of spectral libraries. Papers in related topics (e. g. , photoluminescence and fluorescence microspectroscopy and imaging) will also be considered. |
17. Modulated Structures and Quasicrystals
Organizers: Craig Bennett and Jim Corbett
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Incommensurate modulations and quasicrystallinity represent distinct but related forms of non-periodic long range order. In addition, various levels of disorder in these materials generate unique defect structures, such as discommensurations in incommensurate crystals and phason defects in quasicrystals, which are intimately involved in phase transformations and influence the physical properties. In this symposium, we will highlight recent progress in the application of transmission electron and scanned probe microscopies to the study of these novel materials. |
18. Image Contrast Mechanisms in the Variable Pressure SEM: the ÒNewÓ Imaging DimensionÓ
Organizers: Brendan Griffin and John Mansfield
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Charging has been the bane of SEM users since the instrument's inception. Recently, however, important links between charging and sample state have been recognized and with them, new features have been noted in the images recorded in the VP-SEM. Progress is being to be made in the understanding of these charge-related contrast mechanisms and they have been shown to provide new, and sometimes unique, information on a wide variety of samples, ranging from corn chips to minerals and oil emulsions to doped semiconductors. This symposium will feature invited contributions on new developments in both the applications, control and fundamental theory of charge contrast imaging. Contributions reporting further examples and/or applications of these charge related phenomena are encouraged. Reports of the effects on X-ray analysis in this environment are also sought. |
19. Teaching and Learning Ð Creating Effective, Innovative Solutions in Microscopy, Imaging and Analysis (MIA)
Organizers: Steve Barlow and Ken Baker
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Teaching and learning in the field of microscopy imaging and analysis are important to the practice of all forms of microscopy. Microscopy imaging and analysis has adopted an extensive collection of tools and techniques and continues to develop as an increasingly complex subject area. Computer-assisted microscopies, high speed global internet communications, remote microscopies and image acquisition, digital imaging tools, and multidimensional imaging and analysis techniques all offer opportunities for powerful innovation in teaching and learning. Pedagogical resources include: distance learning, on-line manuals, references, tutorials, image databases, and a selection of shared utilities and dedicated discussion forums. This symposium will encourage contributions that emphasize creative, effective, and innovative educational approaches to microscopy, image acquisition and analysis, and increased use of microscopy and images in a student curriculum. |
20. Elmar Zeitler Symposium: Analytical Electron Microscopy Ð Past and Future
Organizers: Dangsheng Su and Gianluigi Botton
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This symposium is dedicated to Professor Elmar Zeitler on the occasion of his 75th birthday. Elmar Zeitler, a renowned scientist in electron microscopy and the founding editor of Ultramicroscopy, will have his 75 birthday in 2002. This symposium honors his outstanding contributions to the field of electron microscopy. The symposium will feature invited and contributed papers on the general topic of analytical electron microscopy (AEM), including the instrumentation advances and methodological development in EELS, EDX, quantitative TEM, and their applications in biological and materials science. Emphasis will be given to the challenges and future of AEM. |
21. Microstructural Examination and Imagery of Engineering Materials
Organizer: Al Lockley
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Metallographic specimens can be examined using a wide variety of methods involving both traditional and advanced instruments. Several sessions will address applications of optical and electronic microscopy, and advanced analysis tools that can aid the metallographer and materials scientist to understand the microstructure of engineering materials. |
22. Practical Applications of Metallography
Organizer: Al Lockley
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Metallography has been a primary method used to provide engineers and scientist with insight into materials performance, failure, and improvement. Some of the methods used to achieve these insights will be considered including the evaluation of corrosion, failure analysis, and image analysis of engineering materials. |
23. Metallography and Microstructural Evaluation of Contemporary Materials
Organizer: Al Lockley
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As technology advances so do materials and methods to examine them. Several technical sessions will seek to illustrate examples of metallographic preparation and analysis of contemporary engineering materials including light-weight materials, coatings, composites, refractory materials, high-temperature resistant alloys, and steels. |
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Advances in Instrumentation and Techniques |
24. Electron Holography, Interference Phenomena and Related Techniques: A Symposium Honoring the Contributions of Hannes Lichte and Akira Tonomura
Organizers: Vinayak Dravid and Larry Allard
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Electron holography is in a state of transition from the theoretical development and demonstration stage in a few research laboratories to the practical application of the technique in numerous university and industrial laboratories. Holographic and related interference methods in imaging and diffraction have found applications in many materials and biological systems, ranging from semiconductor dopant profiles to contract enhancement and 3-D shape reconstruction of biomolecules. This session will highlight the current state of the art in electron holography research, including new experimental techniques, and applications of holography studies to materials and biological systems. Two pioneering researchers in the field, Prof. Hannes Lichte of the Dresden University of Technology, and Dr. Akira Tonomura of the Hitachi Advanced Research Laboratory, will be honored at this symposium. |
25. Optical Advances in Deep Tissue Imaging
Organizers: Simon Watkins and Peter So
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In vivo imaging of cells and tissues is becoming a critically important research tool, allowing multiple serial data sets to be collected from the same animal, as well as allowing non invasive diagnostic imaging in humans. To maximize the utility of the method, optical tools must be developed which allow deep tissue imaging of molecular and cellular processes. Currently we are at a nexus in which far red illumination systems, bioluminescense, optical coherence tomographic methods and other emergent tools are becoming increasingly realistic approaches to gather high quality images deep within tissues. This symposium will provide a forum to present and discuss these breakthrough in imaging technology. |
26. Aberration Correction in TEM and STEM and its Application to Real-World Materials ¥ A Symposium Honoring the Contributions of Albert Crewe and Harald Rose
Organizers: Max Haider and Ondrej Krivanek
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Aberration of electron lenses has historically been a major limit on the performance of electron microscopes. Following nearly half a century of research in aberration correction that did not produce a practical resolution improvement, successful correctors have now been designed and built for both the TEM and the STEM. This symposium will examine what made aberration correction a reality at last, and illustrate the performance that is now possible with application examples from real-world materials. It will also survey the main challenges of the new Òaberration-correctedÓ era. The symposium will be dedicated to two notable pioneers in the field, Albert Crewe and Harald Rose. |
27. Applications and Developments of Focused Ion Beams
Organizers: Lucille Giannuzzi, Nan Yao and Mike Phaneuf
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The use of the FIB instrument has received much attention in recent years. The imaging, milling, and deposition capabilities of the FIB make it ideal for e. g. , site-specific specimen preparation and nano-machining. Ion channeling contrast allows for imaging of polycrystalline and poly-phase microstructures. In addition, the FIB and dual beam instruments are unique stand-alone analytical tools. Their vast capabilities have enabled numerous applications into the materials sciences and the biological sciences. Abstracts discussing the applications of FIB techniques or the development of FIB instrumentation in either the physical or biological sciences are encouraged to participate in this symposium. |
28. New Developments in Immunolabelling
Organizer: Ralph Albrecht
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This symposium will emphasize emerging labeling technology for use in high resolution and correlative studies. It is becoming increasingly important to identify and localize/co-localize multiple molecular species within cells and tissues. With the development of comprehensive lists of cellular components, the organization and movement of these components at the cellular level is a critical step in understanding cell function. Labels having properties that allow them to be identified in different microscopic modes (LM, EM, FM) are also of considerable advantage, particularly in correlative studies where labeled samples are examined sequentially or simultaneously using several modes. |
29. Industrial Applications of Microscopy Ð Techniques for the Real World
Organizers: Zhigang Li and Janet Woodward
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Microscopic techniques have become more mature and have been broadly used throughout industry. Microscopists from the diverse industrial sectors are invited to share their years of successful and unsuccessful experiences in using various microscopic techniques. Light, scanning and transmission microscopy and associated techniques will be covered in this symposium to illustrate how theses techniques can be combined to solve real world problems. Automation, safety issues, calibration problems, sample preparation, advantages and limitations of the various microscopic technique will be discussed. Another major focus of the symposium will be the evaluation of the new emerging techniques and their potential in industrial applications. |
30. Frontiers of X-ray Spectrometry
Organizers: Dale Newbury and John Henry Scott
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In recent years, advances have been made in X-ray spectrometry with the emergence of the silicon drift detector (SDD), microcalorimeter energy dispersive X-ray spectrometry, and X-ray optics based on polycapillaries. Reports are sought on continuing developments in these and other areas of X-ray spectrometry. SDD with its extremely high count rate raises considerable challenges to MCA binning operations, and we wish to highlight this aspect of SDD development. Additionally, these technologies are now beginning to reach users in the microscopy and microanalysis field, and reports will be welcomed describing the experience of implementing these technologies on electron microscopes and other platforms as well as applying them to practical problem solving. |
31. EELS and EFTEM Analysis
Organizer: Gianluigi Botton and Kevin Moore
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The symposium will focus on new developments in methods, applications and instrumentation of energy loss spectroscopy, energy-loss fine structures and energy filtered microscopy covering both physical sciences and biology. The sessions aim to cover: quantitative aspects of the technique (for spectra and images), novel data analysis approaches (including statistical methods to analyze large data sets), the modeling of energy loss spectra and their limitations (including comparison with complementary techniques such as XAS and XPS), the use of EELS fine structures to understand properties and new instrumentation developments (monochromators, spectrometers, filters and acquisition hardware etc. ). General applications of the technique for microstructural characterization are also welcomed. |
32. Current Topics in Low Voltage SEM
Organizers: David Joy and Raynald Gauvin
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Operation at low- and ultra-low voltage is the most active applications and research field of scanning electron microscopy. This session aims to examine the new and novel forms of instrumentation Ð including parallel projection systems, aberration corrected conventional SEMs, and the point projection microscope Ð which can now offer major improvements in imaging performance at such energies, and the techniques that have been developed for the electron-optical modeling and design of such systems. We will also discuss practical methods for bench marking and verifying the performance of advanced microscopes under realistic conditions. Finally we will have an open Forum on techniques for preparing samples for low voltage microscopy, and on new methods Ð such as plasma cleaning, and CO2 snow Ð for keeping them free from contamination. |
33. Electron Crystallography and Quantitative Electron Diffraction
Organizers: Wharton Sinkler, Yimei Zhu and Jian-Min Zuo
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In recent years, applications of quantitative electron diffraction intensities to structural problems have increased, both in terms of the number problems successfully treated as well as in the range of problems. Examples include direct methods applications to solving inorganic and organic crystal structures, surface structure solutions, structure refinements, investigations of charge state and strain. The potential for more routine utilization of quantitative electron diffraction is great Ð data collection in modern microscopes is fast improving; there are distinct advantages over images in terms of data resolution; and the technique is uniquely suited to beam sensitive and/or nanoscale samples. The challenges range from technical issues of data collection and quantification to robust treatment of dynamical perturbations.
This session will cover all aspects of solving crystallographic or low-dimensional structure problems using quantitative electron diffraction. The main areas will be approaches to the phase problem (e. g. , direct methods), and structure or property refinement from electron diffraction data. |
34. Electron Backscatter Diffraction of Materials: Geology to Nanotechnology
Organizers: John Small and Joe Michael
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Electron backscatter diffraction, EBSD, has emerged as a powerful and important technique for the characterization of a broad range of materials. New generation EBSD systems are being used to acquire and determine crystal orientations for very rapid microstructural characterization of materials. Recent advances in camera technology and identification software have extended the application of EBSD to the determination of the crystallographic phase of unknown materials. Areas of interest include phase identification and orientation studies of nanoparticles, thin films, bulk materials, and fracture surfaces. Also papers are encouraged on the fundamental issues of pattern origin and formation. This session will address recent advances in EBSD instrumentation and software as well as novel applications, which are fueling the current rise in importance of this technique for materials characterization. |
35. Microscopy, Microanalysis and Image Analysis in the Pharmaceutical Industry
Organizers: Bev Maleeff and Barbara Hartman
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Pharmaceutical research and development laboratories are at the forefront of science. Much of the work performed in pharmaceutical microscopy and microanalytical labs is similar to that done elsewhere, however, there are specialized technologies and themes that are of particular value to microscopists in the industry. It is the objective of this symposium to present a variety of biological and materials science applications of significance to the pharmaceutical community. Invited speakers will include current leaders in their respective disciplines. Additionally, in response to feedback from previous meetings, an informal forum will be provided for sharing of thoughts and strategies related to regulatory and other issues faced in our laboratories. Contributed papers for platform or poster presentation on related topics are encouraged and welcome. |
36. Advances in Microwave Technology Ð Creating a Revolution in Biological Specimen Processing for Light and Electron Microscopy
Organizers: Kent McDonald and Rick Giberson
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Microwave-assisted processing reduces the time to prepare samples for thin sectioning electron microscopy from days or weeks to a few hours. For clinical and surgical pathology reduced turn around times have profound implications, and same-day sample evaluations are also immensely useful in teaching and research. In this symposium, the latest results from these areas will be presented. In addition, methods of microwave-assisted immunolabeling for light and electron microscopy, decalcification, in situ hybridization, scanning EM and vital staining techniques for LM will be examined. |
37. Biominerals
Organizers: William Massover and Marija Gajdardziska-Josifovska
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An amazing variety of intracellular and extracellular biominerals are produced by bacteria, plants, and animals. Examples range from skeletal supports or shells based on calcium, silicon, or strontium, through human kidney stones and other clinical deposits, to nano-crystalline iron-rich components of special protein molecules. Their organic nature puts them in the domain of traditional biological electron microscopy, but their inorganic constituent necessitates the use of physical electron microscopy methods.
This joint symposium (biology/chemistry/ geology/physics) aims to present state-of-the-art examples of modern methods for micro- and nano-characterization of normal and pathological biominerals, and to provide a forum for presentation and discussion of original research results. Emphasis will be on structural studies using advanced electron microscopy (including imaging, diffraction, and spectroscopy) as well as with novel approaches by scanning probe microscopies. Contributed reports on interdisciplinary investigations of biominerals are cordially encouraged. |
38. Scanning Probe Microscopy: Technical Advances and Applications
Organizers: Inga Musselman and Dorothy Erie
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This symposium will address scanning probe microscopy of both biological and physical systems. The topics will include advances in imaging techniques and image analysis, as well as the application of the many scanning probe microscopy techniques including, among others, scanning tunneling microscopy, atomic force microscopy, scanning electrochemical microscopy and near-field scanning optical microscopy. |
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Technologists' Forum Symposium: |
39. Special Staining Techniques for Biological/Materials Samples
Organizer: Jeanette Killius
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This symposium will deal with specific stains or etches to delineate particular areas in various samples. The emphasis will be on exploring novel stains, staining to enhance a previously unseen area, proper protocol, overcoming problems and imaging. Materials samples such as polymers, semiconductors and papers/textiles will be discussed. Immunostaining, fluorescence, and alternative EM stains are among biological applications to be presented. Invited papers will introduce these areas. Contributions for poster presentations covering this topic will be welcome. |
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Corporate Session: |
40. Corporate Members Session
Organizer: Mike Kersker
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This session will consist of contributed presentations by our corporate members and exhibitors at the meeting and will provide the opportunity for them, or researchers using their products, to present information concerning recent technological and application oriented material as platform and poster presentations. |
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Additional Categories for Contributed Papers |
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Applications of Microscopy and Microanalysis: Biological Sciences |
41. Biological Microanalysis
42. Biological Ultrastructure (Cells, Tissues, Organ Systems)
43. Biomedical Applications
44. Biopolymers and Biomemetics
45. Blood/Immunology
46. Botany
47. Correlative Microscopy
48. Developmental/Reproductive Biology
49. Entomology
50. Microbiology
51. Neurobiology
52. Parasitology
53. Pathology |
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Applications of Microscopy and Microanalysis: Physical Sciences |
54. Advanced Composites
55. Films/Coatings
56. Geology/Mineralogy
57. Ferroelectrics
58. Modulated Structures and
59. Oxidation/Corrosion
60. Phase Transformation in Metals, Alloys and Ceramics
61. Radiation Effects in Materials
62. Semiconductors
63. Specimen Preparation for Materials Sciences
64. Surfaces/Interfaces |
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Advances in Instrumentation and Techniques |
65. Auger Electron Microscopy
66. Bright Field and Fluorescence Microscopy
67. Compositional Mapping
68. Computational Methods for Microscopy and Microanalysis
69. Cytochemistry (Light and Electron Histochemistry, Immunohistochemistry, In-Stu Hybridization)
70. Diffraction Techniques
71. Electron Crystallography
72. Electron Holography
73. Field Ion Microscopy 7
74. High Resolution Electron Microscopy
75. Image Simulation and Image Processing Techniques
76. In Situ Microscopy Techniques
77. Instrument Performance
78. Live Cell Imaging
79. Microbeam Mass Spectroscopy
80. Molecular Spectroscopy
81. Secondary Ion Mass Spectroscopy
82. SEM
83. Stereology
84. XRF/XRD Techniques
85. X-ray and Optical Crystallography |
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Tutorials |
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Organizers: Gina Sosinsky and Ian Anderson
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Tutorial lectures are in-depth reviews of new or evolving technologies of interest to microscopists. They take place during the meeting and are designed to provide an introduction to the field and its application. No prior knowledge of the field is assumed and ample time is provided for questions and discussion. Most tutorials are videotaped for inclusion in the MSA video library. Tapes are available for purchase from the MSA Education Committee. |
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Biological Sciences Tutorials |
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86. Dissecting Molecular Structure and Function of Complex Biological Systems: Selected Topics (86-89)
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In this era of proteomics where protein structures once thought of as complex are being determined to atomic resolution, another challenge for structural biology is obtaining, analyzing, and integrating protein structures into macromolecular assemblies at complexity levels ranging from organelles to tissues. Often this involves using complementary imaging and image reconstruction techniques. This year's biological tutorial session contains three different microscopy approaches, (1) cryo-EM of viruses and other large macromolecular complexes, (2) AFM of macromolecular complexes performed under near-to-native hydrated conditions and (3) tomographic reconstruction of cells and organelles. These tutorials are aimed at describing state-of-the art techniques for determining the structure-function dynamics in higher level organizations but at molecular or sub-molecular resolutions. Each method provides three-dimensional information about the spatial or temporal arrangement of the constituent molecules within the context of the larger ensemble. |
87. Cryo-electron Microscopy of Large Complexes: A Cold Look at Fusion
Speaker: Stephen Fuller |
88. Cellular Dynamics Observed at Sub-nanometer Resolution Using Atomic Force Microscopy
Speaker: Daniel Mueller |
89. The Nuts and Bolts of Electron Tomography: 3D Imaging of Big and Messy Biological Structures
Speaker: Gina Sosinsky |
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Physical Sciences Tutorials |
90. Spectral Imaging: Getting the Most from All that Data
Speaker: Paul Kotula
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How do you comprehensively analyze the chemistry of a microstructure? One might do a series of point analysis or perhaps acquire a series of elemental maps. Both of these have the potential to miss important features of the microchemistry. An alternative method would be to combine the best of both point analysis and mapping by acquiring spectral images. Spectral images, where a complete spectrum is collected from a 2D array of pixels, contain an enormous amount of data of interest to microanalysts but the problem is how to get at it. Current personal computers, given the right direction, are powerful enough to take on the challenge posed by such large data sets. The focus of this tutorial will be to review and evaluate several different methods for analyzing spectral images from techniques such as SEM/STEM X-ray and EELS, WDS, time-of-flight SIMS, etc. Among the spectral image analysis methods discussed will be, filtering, mapping and multivariate statistical analysis. Some of the issues that will be addressed include microstructural sampling, acquisition conditions and artifacts, processing of spectral image data for analysis, and interpretation of the analytical results. |
91. Electron Backscatter Diffraction in the SEM: Orientation Mapping and Phase Identification for Materials Science
Speaker: Joe Michael
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Electron backscatter diffraction (EBSD) is becoming a standard tool for the microstructural characterization of crystalline materials. One of the big disadvantages of SEM characterization has been the lack of a robust way to obtain crystallographic information about the specimen. The development of EBSD has overcome this disadvantage. EBSD is now routinely used for both orientation mapping and phase identification. This talk will start with a general introduction to EBSD in the SEM. The history of the technique and the origin of EBSD patterns will be presented followed by a discussion of practical considerations in the application of EBSD. Automated orientation determination will be introduced and illustrated with examples from a variety of materials. The development of EBSD for phase identification will be discussed and illustrated by example. |
92. Quality Systems for Microscopy & Microanalysis: ISO 9000 and More
Speaker: Eric Steel
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How do you judge the performance of your microscopy and microanalysis lab? How do you know that you are giving the correct answer? Learn what other microscopists and microanalysts are doing to assure the quality of their results. Quality standards for ISO 9000, QS9000, Six Sigma, and ISO 17025, among others, have many things in common. These include a system for the quality of staff, laboratory, measurement methods, equipment, sampling, and reporting results. Aimed at the physical sciences, a discussion of internal approaches and documentation will be given by a group of professionals who have experienced it first hand. The discussion will also include approaches to external evaluation and recognition of performance systems, such as interlaboratory round robin/proficiency testing, external auditors, and accreditation. |
93. Scanned Probe Microscopy
Speaker: Phil Russell
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This tutorial will introduce the basic concepts and operating modes of Scanned Probe Microscopy (SPM), including scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The SPM techniques most commonly utilized today are based on the basic AFM concept, and include variations of both contact mode, which is the original and most simple operating mode of AFM, and oscillating tip modes, which are also known as AC, non-contact, intermittent contact or tapping modes. The tutorial will begin with a description of the basic instrumentation common to all SPMs, followed by more detailed descriptions of each of the more common modes of operation, with the particular advantages of a given mode illustrated by typical applications. The tutorial will include a discussion of the role of SPM in a microscopy and microanalytical laboratory, addressing the commonly asked question: ÒDo I need AFM if I already have SEM and/or TEM?Ó |
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Joint Physical and Biological Sciences Tutorials |
94. Practical Methods for Transmission Electron Microscopy of Polymers
Speaker: Bob Vastenhout
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Morphological characterization of polymers and polymer blends by transmission electron microscopy (TEM) is complicated by the low intrinsic level of available contrast. This tutorial will cover practical methods for TEM contrast enhancement of polymer microstructures, including ultramicrotomy and staining. Among the topics to be discussed are: factors affecting the choice of temperature at which ultramicrotomy is performed (e. g. , room temperature or cryo?), and sectioning artifacts; various methods of contrast enhancement, the relative advantages and disadvantages of block staining as opposed to the staining of ultra thin sections, specific staining agents (e. g. , OsO4, RuO4), double staining of polymer blends, and staining artifacts; and problem solving strategies. Technical details and practical tips will be covered. |
95. Basic Confocal Microscopy
Speakers: Jay Jerome and Bob Price
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Confocal microscopy has become a primary method in many laboratories for visualizing microscopic structure in three dimensions. The material in this tutorial will be aimed at beginning and intermediate level users and will cover the basic components in a confocal system (lasers, dichroic mirrors, microscope objectives, pmt's etc) and will address many of the concerns that a researcher must be aware of in properly setting up the user adjustable parameters to optimize image collection. In addition, we will also discuss some basic considerations for the preparation of specimens (fixation and fluorescent staining), digital imaging and 3-D reconstructions that are essential in obtaining optimum results with a confocal system. Although the emphasis of the discussion will be on laser scanning confocal systems, information on other types of systems, including spinning disk microscopes, will be presented.
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