Full Symposium Descriptions

Instrumentation & Techniques Symposia
A1 Oliver Wells Memorial Symposium on the Scanning Electron Microscope

Lynne M. Gignac, David C. Joy, Brendan J. Griffin

Dr. Oliver C. Wells was one of Sir Charles Oatley’s first students to work on the scanning electron microscope (SEM) at Cambridge University and is widely considered one of the pioneers of the commercial SEM. For most of his 60 year career, Dr. Wells studied various aspects of the SEM but had particular interests in “low loss”, energy filtered and very high energy (>100 keV) SEM imaging, SEM electron detector design and practical applications, and studying ways to reduce charging in SEM. Dr. Wells passed away in 2013 and this symposium is intended to honor his memory and legacy by soliciting papers on research, development, history and uses of the SEM with a particular emphasis on his research interests.

A2 Advances in Imaging and Spectroscopy in STEM

Nigel D. Browning, Peter D. Nellist, Maria Varela del Arco

In the 25 years since the first demonstration of atomic resolution Z-contrast imaging, STEM has risen from a technique performed only at a select few institutions to a ubiquitous method for atomic scale imaging and analysis. The aim of this symposium is to provide a forum to discuss the key developments that brought us to this stage and how the lessons learned from along the way can enhance the future development and integration of STEM methods. Contributions are encouraged that concern all aspects of the use of STEM - including instrument and technique development for all forms of imaging and spectroscopy and their application to in-situ analysis. Advanced applications of these approaches to solid state materials, nanostructures and biological systems are particularly welcomed.

A3 TEM Phase Contrast Imaging in Biological and Materials Science

Michael Marko, Radostin Danev

Conventional TEM phase-contrast imaging limits maximum information transfer to a narrow band of spatial frequencies. This can be avoided by in-focus imaging with a physical phase plate. The theory, construction, and practical use of phase plates will be explored. In biological cryo-TEM, high-contrast, high-resolution imaging at low electron dose is facilitated. In materials science, the combination of a physical phase plate with tunable Cs offers an unparalleled opportunity for characterization of both atomic details and larger structures. The number of laboratories exploring the use of phase-plates is growing, and this will be a timely opportunity to learn from each other.

A4 Electron Holography at the Atomic Scale and the Nanoscale

Molly McCartney, David J. Smith, Lin Zhou

Electron holography enables convenient access to the phase and amplitude of the electron wave passing through the TEM specimen, allowing both atomic-scale imaging as well as quantitative measurement of nanoscale electrostatic and magnetic fields. This symposium will consider recent advances in electron holography techniques and applications. Platform and poster presentations will include the emergence of novel approaches and instrumentation for electron holography, as well as providing an overview of latest applications to piezoelectric and ferroelectric materials, measurement of charge, magnetic nanostructures, both natural and man-made, and dopant profiling in semiconductor devices.

A5 15 Years of Focused Ion Beams at M&M

Lucille A. Giannuzzi, Keana Scott, Nicholas Antoniou

The first FIB session at M&M 2000 consisted of 20 papers and was dominated by applications of Ga+ focused ion beams for TEM specimen preparation. Since then, the use of FIB-based instruments has expanded to 3D analysis, specimen preparation for atom probe tomography and other techniques, nanomachining, and nanodeposition for inorganic materials, organic materials, and biological materials in ambient and cryo conditions. In addition, the development of new ion sources beyond LMIS to GFIS and ICPS using e.g., He+, Ne+, Xe+, and other ions have allowed unique imaging, analytical, milling and deposition capabilities while expanding usable length scales. Papers are encouraged on topics of focused ion beams including instrumentation, theory, and applications.

A6 Super Resolution Microscopic Methods

Angus I. Kirkland, John M. Rodenburg

This symposium will cover the development and applications of super resolution methods in the spatial and temporal domains, using all radiations, including electrons, X-rays and photons. Super resolved methods include developments and applications that use novel experimental dataset geometries in image and diffraction space to extend resolution and the computational methods required to extract and process the information from these. In particular, contributions that describe methods that are generally applicable to multiple radiations in a correlative fashion are welcomed as are applications of these methods to the life and physical sciences.

A7 Microscopy and Spectroscopy for Power Generation and Energy Storage

Eva Olsson, Wolfgang Jaeger

Among our most important challenges are reduction of energy consumption and environmentally friendly power generation and conversion. The performance of materials for energy technology is determined by their structure. This symposium will cover aspects of structure, properties and life-cycle analysis of materials for power generation, energy conversion, renewability, storage and reduced energy consumption. The topics include microscopy and spectroscopy investigations including techniques of Cs aberration corrected electron microscopy, monochromated spectroscopy and in situ microscopy of photovoltaics, nuclear technology, fuel cells, ion conductors, energy storage, thermoelectrics, correlation between synthesis, structure and properties and specimen preparation for microscopy studies.

A8 Nano-Characterization of Emerging Photovoltaic Materials and Devices

Robert F. Klie, Moon Kim

The efficiency of solar cells and other energy-conversion devices can be further improved if detailed information of how defects behave optically and electronically can be obtained. Aberration-corrected (scanning) transmission electron microscopy is one the most versatile experimental techniques to explore the structure-property relationships of materials at the nanometer and atomic scale. This symposium will focus on analytical transmission electron microscopy techniques, aberration-correction, monochromated energy-loss spectroscopy, and in-situ methods to characterize energy conversion materials. Presentations are sought from the areas of polycrystalline single-junction solar-cells, next generation multi-junction approaches, superlattice or quantum-dot thermoelectric devices.

A9 Frontiers in Analytical TEM-STEM

Gianluigi Botton, Juan Carlos Idrobo, Ai Leen Koh, Paolo Longo

With recent advancements in electron microscopy, fast spectroscopy detectors, and software for efficient acquisition and analysis of the data acquired, physical and chemical properties of materials can be now explored with unprecedented spatial and spectral resolution. This symposium aims to bring together scientists working at the forefront of spectroscopy in the scanning transmission electron microscopy (S)TEM. The symposium will showcase hardware and algorithmic improvement in EELS, EFTEM, EDS and cathodoluminescence spectroscopy and their applications to both materials and biological sciences. Of particular interest are studies of low-energy transitions, quantitative compositional mapping, fast chemical state mapping and how these data relate to physical properties of the materials.

A10 X-ray Imaging

Jeffrey M. Davis, Eric Telfeyan, Richard Wuhrer

This broad ranging symposium will cover all aspects of X-ray imaging in the SEM, electron microprobe, TEM, µXRF, and synchrotron based methods. We are actively seeking contributions detailing new detector technology, new software, and innovative methods for analyzing complex materials. The goal of this symposium is to bring together a diverse group of experts who can present on everything from hyperspectral data analysis techniques to large area X-ray imaging of cultural heritage objects. We are also actively seeking contributions from students and young professionals interested in presenting new and innovative research.

A11 Frontiers of Electron-Probe Microanalysis

John Armstrong, Paul Carpenter, Hideyuki Takahashi, Mike Jercinovic

EPMA continues to see exciting developments such as FE-EPMA analysis at high resolution and low voltage, applications of SDD EDS and WDS to quantitative analysis, quantitative analysis via hyperspectral x-ray mapping, chemical state determination using low energy x-ray lines, improved automation and instrumental capabilities, and improvements in correction algorithms. This session will highlight invited presentations from the international community. We welcome contributed presentations from the scientific, vendor, and student communities, and encourage presentations on new technologies, problem solving, and practical solutions to analytical problems.

A12 3D Imaging and Microanalysis: Image Analysis and Applications

Paul G. Kotula, Keana Scott

Recent advances in detector technology, microscopy instrumentation and computing resources enable routine 3-dimensional structural, elemental and chemical imaging of materials, providing unexpected insights and better understanding of biological processes and material properties. However, advances in 3D imaging and microanalysis also introduced challenges specific to 3D analysis including multi-dimensional data processing and management problems. In this symposium, we will focus on the new methods and approaches in 3D imaging and microanalysis, novel 3D analysis applications, and data processing topics such as computational, mathematical and computer vision methodologies for characterization of 3D structures in physical and biological sciences.

A13 Practical Applications and Analytical Trends of Metallography and Microstructure

Frauke Hogue, Frank Mücklich

This symposium will draw together metallographers, engineers and researchers working on a wide range of materials produced by many different methods. The session will focus on efforts to reveal and characterize the true microstructure, with a special emphasis on deformation studies. Topics include advances in equipment and consumable technology that enhance the ability to prepare materials so that the true microstructure is revealed. Additionally, this session will address the application of these techniques to both research and industrial samples.

A14 Advances in Cathodoluminescence and Soft X-ray Microanalysis

Colin M. MacRae, Marion A. Stevens-Kalceff, Scott A. Wight

Cathodoluminescence (CL) and soft x-ray microanalysis provide unique high sensitivity information about defect microstructure, bonding and trace chemistry. CL is experiencing a resurgence due to improvements in instrumentation, data analysis, while soft x-ray microanalysis is a rapidly emerging field. With improvements in SDD technology and new WD based spectrometry the detection of ultra-soft x-rays (Li-Ka) is now possible. Challenges include surface preparation, detector stability and difficulties in dealing with peaks that reveal the complexity of the Density of States. CL and ultra-soft x-ray detection require a combined approach of measurement and quantum mechanical modelling to interpret both composition and structure. Contributions covering theory and applications are encouraged.

A15 Cs-Correctors: Current State and Ongoing Developments

Max Haider, Rolf Erni

Nowadays, more than 400 spherical aberration correction systems have been installed worldwide and are used frequently for high resolution transmission electron microscopy. This symposium will concentrate on discussions of various aspects of such systems, e.g., the measurement of aberrations and their long-time stability, advanced applications like Lorentz microscopy and holography, etc. The different correction systems and the latest developments as well as the now understood resolution limits of aberration corrected imaging and how to tackle them should be discussed.

A16 Correlative Microscopy and Microanalysis from Macro to Pico

Brian P. Gorman, Christoper J. Gilpin, Mor Baram

Correlative microscopy is where the same sample is analyzed using two or more techniques that typically differ in scales ranging from the macroscopic to the atomic level. The resulting combined data sets can be used for both materials and life sciences in a variety of circumstances, e.g., localization of rare events, determining if small regions are typical of the bulk, and combining imaging and analytical techniques. Examples include optical imaging before FIB specimen preparation, combined SEM and SPM and fluorescence-labeled imaging before TEM specimen preparation, among others. This symposium solicits presentations of multi-scale imaging, analysis and/or innovative correlative technique or instrumentation development in both the physical and biological sciences.

A17 Extended Crystal Defects: Quantification of Strain, Local Atomic Structure and Chemistry

Douglas L. Medlin, Jim Ciston, Yoosuf N. Picard

Extended crystal defects such as dislocations, stacking faults, and grain boundaries strongly influence materials properties and behavior. Our fundamental understanding of such defects has benefited from decades of advances in transmission electron microscopy, while new developments in atomic resolution microscopies, three-dimensional imaging, and diffraction contrast methods are continuing to invigorate the field. This symposium will focus on the application of advanced microscopic methods to the fundamental study of extended crystal defects in materials. We welcome submissions investigating extended crystal defects across the full range of materials classes.

A18 Vendor Symposium: New Tools for Life and Materials Sciences

Alice C. Dohnalkova, Elizabeth R. Wright, Mark A. Sanders

This symposium provides an opportunity for instrument manufacturers and vendors to showcase new developments resulting in improved technology solutions. Topics include: new methods and techniques; new developments and technologies; breakthrough and new instrumentation; and improvements to existing instrumentation.


Biological Sciences Symposia
B1 Dr. Gerard Simon Memorial Symposium on Anatomic Pathology

Sara E. Miller, Pierre-Mathieu Charest

Dr. Gerard Simon, a renowned anatomical pathologist and ultrastructural researcher, specialized in Laboratory Medicine at the University of Geneva, and studied under Dr. Kellenberger in Pathology and Dr. Rouiller in Histology. In 1967, he became EM Laboratory Director at the Banting Institute and in 1979, the EM Laboratories Director at the Faculty of Health Sciences, McMaster University, where he helped found the Canadian Centre for EM. One of the founders of the Microscopical Society of Canada and the Canadian Foundation for the Development of Microscopy, he is considered as a major pioneer in the development of microscopy in biological sciences in Canada. This symposium will concentrate on Anatomic Pathology and Diagnostic EM and will dovetail with the Diagnostic EM FIG program.

B2 Microbes and Microbial Communities

Elizabeth R. Wright, Teresa Ruiz, Gary Dunny

Our understanding of the structure and function of microbes and microbial communities has advanced significantly with the application of EM techniques and correlative methodologies. This symposium highlights structural and ultrastructural studies of bacteria, archaea, fungi, protists, and viruses using electron microscopy techniques singly or combined with other structural methods. Topics will include: microbe architecture and function; microbe-host interactions; species diversity in microbial communities; microbial community structure and function; and interactions, signaling, and sensing in microbial communities.

B3 Nuclear Architecture and Chromatin Structure: 40 Years after the Nucleosome

Ada L. Olins, Donald E. Olins

Nuclear architecture is dynamic in 3D organization and composition. Cycling cells must continually and repeatedly reconstruct the interphase nucleus. Epigenetic markers, associated proteins, RNA and lipids modify the fundamental nucleosomal “string of beads” structure. How do these modifications influence higher order organization, including polynucleosome folding, chromatin domains and chromosome territories? This session will explore innovative electron microscopy methods and high-resolution light microscopy, which augment genetic and biochemical information, in an effort understand nuclear architecture at all levels. Young people with fresh ideas are encouraged to participate.

B4 Advances in Sample Preparation for Cryo-EM Studies

Isabelle Rouiller, Howard Young

Electron cryo-microscopy (cryoEM) is an exciting technique to understand the function of macromolecular complexes. This symposium highlights advances in preparation methods to address the challenges of studying flexible complexes and to allow the study of complexes in native membranous environments (using detergents, nanodiscs, amphipols and other systems, either by single particle or two-dimensional crystallization methods) or cellular environments (using cryo-sectioning, cryo-milling/FIB and correlative light and electron microscopy). Topics will include applications in a range of subjects of molecular and cellular biology, such as eukaryotic and prokaryotic architecture, cell division, protein expression, cellular signaling, and host-pathogen interactions.

B5 Structural Biology and Ultrastructure

Michael Radermacher, Paula da Fonseca, Ingeborg Schmidt-Krey, Caroline Miller

Recent developments on EM methodologies have greatly enhanced our understanding of the 3D structure and function of biological systems. This symposium highlights structural and ultrastructural studies of cells, microorganisms and macromolecules using electron microscopy techniques (e.g. single-particle analysis, tomographic methods; helical reconstruction, crystallographic methods) singly or combined with other structural methods (e.g. X-ray methods; atomic force microscopy). Topics will include: structure and function of macromolecular assemblies, virus structure and virus-host interactions; eukaryotic and prokaryotic cell architecture; cellular metabolism; cell division and protein translation; cellular secretion, adhesion and motility; cell-cell communication and signaling.

B6 Microanalysis of Biological Materials

Peta L. Clode, Richard D. Leapman

This session will encompass all aspects of elemental and isotopic microanalysis of cells, tissues and biominerals across electron, ion and X-ray platforms. Topics will focus on advances in instrumentation, as well as novel methods for sample preparation and data analysis. Techniques will include: energy-filtered TEM (EFTEM), electron energy-loss spectroscopy (EELS), electron probe x-ray microanalysis, x-ray nanoprobe analysis, and secondary ion mass spectrometry (SIMS). Papers will also cover a wide range of applications from the biomedical sciences to basic biology, as well as agricultural and plant sciences, and bionanotechnology. Submissions are encouraged from experts through to students, with the view to inspiring interaction between biologists and microanalysts.

B7 Light Sheet and Multi Photon Imaging

Peter Santi, Thomas Stroh

Fluorescence microscopy provides high contrast and specificity imaging, while being compatible with live imaging. The environment of a cell is crucial for many biological processes and disease development. Whole organism and in vivo models as well organ cultures are highly suitable to elucidate complex mechanisms underlying these processes. However, there size and tissue density represent a special challenge for light microscopy. This session will discuss how multi-photon and light sheet microscopy address these issues. Applications adapted to time-resolved, live imaging and especially in the context of highly dynamic processes will be presented.

B8 Optical, Confocal, and Floresence Imaging

Judith Lacoste

The instrumentation for conducting microscopy-based experiments has developed significantly over the last years. Indeed the diffraction limit was broken by different optical and computational methods. It remains however that microscopy is not just about an instrument. The sample of interest needs to be prepared according to the goal of the research and the type of imaging required. This session will discuss recent advances in the development of fluorescent probes and tissue clearing techniques. These new tools, in combination different microscope modalities, allows for significant improvement in the ability of researchers to image more challenging samples such dynamic processes and deep tissues.

B9 Utilizing Microscopy for Research and Diagnosis of Diseases in Humans, Plants, and Animals

W. Gray Jerome, Patricia E. Kysar, Michael P. Goheen

Microscopy is not only useful but critically important in the ongoing research, detection, diagnosis and treatment of disease. Advances that improve rapid and accurate detection and treatment often involve the use of various microscopic techniques. These varied techniques provide us with an improved ability to diagnose and research the origins, development and response of diseases in human, plant and animal specimens. This is an opportunity to share information on the investigation of pathogenic cells, tissues and entire organisms in clinical, diagnostic and research laboratories. Emphasis will be placed on both rapid detection and improvements in methodologies.

B10 Microscopy, Microanalysis and Image Analysis in the Pharmaceutical Sciences

Lynn DiMemmo, Alejandra Camacho

Pharmaceutical research and development presents unique challenges that have led to the development of highly specialized analytical methods. This symposium will present applications of microscopy associated techniques to biological and materials science problems that arrive during drug discovery, vaccine research, formulation and production. In addition to presentations by invited speakers, an informal forum will be provided for sharing of thoughts and strategies related to regulatory, throughput, archiving and other issues faced in our laboratories. Contributed papers for platform or poster presentation on related topics are also welcome.


Physical Sciences Symposia
P1 Analytical Techniques and Their Application for the Study of Deformed Microstructures

Michael B. Matthews, Frederick Meisenkothen, Stefan Zaefferer

This symposium will draw together technicians, engineers and researchers who are working in fields related to the study of deformation of materials. The session will focus on the latest efforts within the microscopy and microanalysis community to study structure-mechanical property relationships and to reveal and characterize the true microstructure produced in materials during deformation. Included within this focus are progress and advances in instruments, equipment, methodologies, and analytical and computational tools. Additionally, this session will address the application of these developments to both research and industrial samples. We invite and welcome contributed presentations from the scientific community. A special emphasis will be made to highlight student research.

P2 Advances in In-situ Microscopy

David A. Muller, Haimei Zheng, Adam P. Hitchcock, Thomas LaGrange

In situ electron and x-ray microscopies are playing an increasingly important role in addressing key scientific questions in materials science, chemistry and biology. The aim of this symposium is to provide a forum for scientists from diverse fields to discuss the latest advances in high-resolution in-situ microscopy. Areas of interest include but are not limited to environmental, dynamic, electrochemical cell TEM/STXM, in situ straining, in situ x-ray imaging, microdiffraction, etc. Topics on technical developments, such as specialized holders, detectors and microscopes, data quantification for in-situ experimentation, or applications of in-situ methods for catalysis, corrosion, batteries, material transformations, nanoparticle nucleation and growth, and biological processes are welcome.

P3 Mineral Analyses from Laboratory to Spacecraft

Rhonda M. Stroud, Zack Gainsforth

Mineral analyses can provide both economically valuable information regarding natural resources, and scientifically vital information regarding the formation and evolution of the solar system. The technical requirements for these analyses frequently push the limits of microanalysis. Current challenges include: How much chemical, structural, and isotopic information can be obtained from a single nanoparticle? With what certainty can a mineral mixture be identified with field or spacecraft instrumentation? What does the 3D microstructure reveal about a sample’s geological history? Papers are solicited that address technical advances in microanalysis methods and/or novel applications of established methods for Earth and planetary materials research.

P4 Carbon Nanomaterials and Related Counterparts: Recent Results and Challenges

Raul Arenal, Kazu Suenaga

Fullerenes, nanotubes, nanodiamond and graphene are promising nanostructures for potential applications due to their unique properties. Most of these nanostructures are pristine carbon materials, but there is also a significant interest in related carbon-doped nano-objects and counterparts, e.g., layered materials such as BxCyNz, WS2 or MoS2. TEM techniques have provided major advances in the study of these materials, including at the atomic scale by aberration corrected TEM. This symposium will focus on the state-of-the-art, current challenges and perspectives of TEM studies in these materials. Studies employing other characterization techniques, including Raman and infrared spectroscopy, XPS, photoluminescence, cathodoluminescence and scanning probe microscopy are also welcomed.

P5 Microanalysis of Irradiated Materials: Preparation, Instrumental Development, and Analysis

Karen E. Wright, Olivier Dugne, Philipp Poeml, Adam Robinson

Understanding the properties and behavior of nuclear materials as components or fuels throughout all stages of the fuel cycle (as fabricated, in-pile, accidental conditions or long-term storage conditions), requires a set of thermomechanic, neutronic or thermodynamic modeling tools. Data for such modeling arises from the microanalysis of these materials, which requires complex analytical techniques and equipment. Such techniques and equipment are difficult to develop, test, and implement, particularly when handling irradiated materials. This symposium will highlight approaches to addressing these challenges including sample preparation techniques, analytical approaches, facility and equipment set-up and modification, modeling, and data interpretation.

P6 Failure Analysis of Structural Materials: Microscopy, Metallography and Fractography

Daniel P. Dennies, Ronald J. Parrington

This symposium is intended to be a forum for the exchange of information and knowledge regarding the use of microscopy, metallography and fractography in structural materials-related failure analysis. Invited papers would include those involving failure investigations where microstructures, metallography and fractography are critical to identifying the root cause. Of particular interest are unique, innovative, and/or challenging applications of microscopy, metallography, fractography, and sample preparation in failure analysis for industrial or research applications. Target attendees will include engineers and scientists from all levels of analytical expertise and all related backgrounds, not just materials engineers.

P7 Microscopy and Characterization of Ceramics, Polymers, and Composites

S.K. Sundaram, James E. Martinez

Compositions, fabrication processes and applications are continually under development in the ceramic, plastic, and coatings worlds with the aid of conventional imaging of microstructures and experimental analysis. Emerging imaging techniques often reveal mechanisms and other details of these developments, especially when at least one dimension is on the micro or nano scale. This session showcases what is new in ceramography, plastography and coating analysis.

P8 Imaging and Analysis of Cultural Heritage Materials

Edward P. Vicenzi, Marc Walton, Loïc Bertrand

Cultural heritage researchers have benefited enormously from a number of recently developed and significantly improved imaging and analysis capabilities. Topics in this symposium will cover a range of methods used to characterize materials, from cutting-edge tools for the examination of ultra-precious and one-of-a-kind objects, to proven imaging and microanalysis techniques for documenting specimen condition and alteration. Contributions from researchers, conservation scientists, conservators, and students interested in applications to cultural heritage are welcome.

P9 Surface & Subsurface Microscopy & Microanalysis in Materials & Biological Systems

Vincent S. Smentkowski, John A. Chaney, Chanmin Su

Surface properties (composition, uniformity, thickness, topography, etc) dictate the performance of many materials and biological systems. The surface analyst is asked to detect & image species present in ever-lower concentrations & within ever-smaller spatial and depth dimensions. This symposium will emphasize state of the art surface analytical instrumentation including all aspects of surface mass spectrometry and scanning probe microscopy including nano-scale chemical analysis via TERS and IR; advanced data analysis tools; the use of complementary surface analytical instrumentation to perform a complete analysis of complex materials &/or biological systems; and surface analytical challenges. Contributed papers on surface analysis are solicited for both platform and poster presentations.