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The physical sciences and biological sciences tutorials serve mainly as an educational tool for attendees of the annual Microscopy & Microanalysis meeting by affording a select number of researchers to give extended lectures on the practical aspects of certain microscopy techniques, methods, and computations. Generally focused on cutting-edge and/or immediately relevant microscopy, the tutorials give speakers the opportunity to venture well beyond the cursory introductory material of a platform presentation, which provides attendees with an in-depth and practical understanding of a given technique.

X40 Physical Sciences Tutorial:
ABF STEM - Direct and Robust Atomic-resolution Imaging of Light Elements in Crystalline Materials

Scott Findlay

Annular bright field (ABF) imaging in atomic-resolution scanning transmission electron microscopy has been shown to give direct, robust and real-time imaging of light elements in crystalline materials. Columns of lithium, even hydrogen, can be imaged in some circumstances. This brief tutorial will promote the usefulness of the technique. Practical aspects will be emphasized, in particular what specimen characteristics and experimental parameters are most suited to this imaging mode. The conceptual underpinnings and limitations of ABF imaging will be discussed, drawing from a mix of exploratory simulations and experimental results. Strategies for implementing ABF imaging and recent extensions of the technique will be described.

X41 Physical Sciences Tutorial:
In situ Liquid S/TEM: Practical Aspects, Challenges, and Opportunities

Raymond R. Unocic

Recent advances in platforms for in situ/operando liquid S/TEM experimentation has brought forth new research opportunities in the physical and life sciences, where it is now feasible to image static and dynamic processes of materials in their native liquid environment, at high spatial resolution, and under an external stimuli. In the context of performing quantitative in situ experiments and across this progressively growing research field, there is a need to understand complex electron beam interactions, recognizing and separating out experimental artifacts, coupling analytical microscopy methods for chemical analysis, performing electrochemical measurements for electrochemistry, data collection and interpretation.

X42 Biological Sciences Tutorial:
Maximizing the Likelihood of Successful Maximum Likelihood Classification

Dmitry Lyumkis

Maximum likelihood methods are being increasingly used in three-dimensional electron microscopy to determine the structures of challenging biological molecules. This tutorial will feature a brief introduction to maximum likelihood methods and will discuss best practices for performing maximum likelihood classification of EM data.

X43 Biological Sciences Tutorial:
Advances in Light Sheet Microscopy

Liang Gao

Light sheet microscopy is an up-and-coming technique in light microscopy as it offers reduced photobleaching, highly efficient image recording, and significant depth penetration. This tutorial will feature an introduction to the technique and will discuss new developments and results that the technique has enabled.

X44 Biological Sciences Tutorial:
Optimizing Specimen Preparation for Macromolecular Electron Microscopy

Deborah Kelly

Specimen preparation can one of the most challenging steps for macromolecular electron microscopy. This tutorial will discuss some new approaches for preparing optimal samples for three-dimensional electron microscopy.