Physical Sciences Tutorials

X40 - Advanced Cryo-FIB Specimen Preparation and Handling of Environmentally Sensitive Materials for APT and TEM Analysis

Daniel Perea, Pacific Northwest National Laboratory

Dr. Perea is a senior staff scientist at the Environmental Molecular Sciences Laboratory at the Pacific Northwest National Laboratory. He received his Ph.D. in materials science & engineering from Northwestern University in 2009. Dr. Perea has over 14 years of experience using atom probe tomography, with much of his expertise in semiconductor and structural materials. His current interests at PNNL include developing unique cryogenic-based hardware and protocols to pioneer the use of atom probe tomography to probe the composition and structure of hydrated materials.

X41 - Entrepreneurship in the Microscopy Community

Benjamin Bammes, Direct Electron

Benjamin has a broad scientific background, including physics, computer science, biology, and chemistry, working with small businesses related to science since 2003 in addition to academic research. He has a Ph.D. in Molecular Biophysics from Baylor College of Medicine, where he worked under National Academy of Sciences member Dr. Wah Chiu to optimize cryo-EM data collection and image processing. In 2011, Benjamin joined Direct Electron and now leads our collaborative approach to delivering high-tech solutions to the scientific community.

Several entrepreneurs from the microscopy community participate in an open Q&A forum on topics related to starting and running a microscopy-related business. Topics may include:

X42 - TriBeam Tomography for 3D Data Acquisition

McLean Echlin, University of California-Santa Barbara

McLean Echlin is a research scientist at UC-Santa Barbara where his interests are in problems related to 3D materials science, strain mapping, and other advanced characterization techniques that are applicable at the mesoscale. Over the past decade he has developed multiple iterations of the femtosecond laser enabled TriBeam microscope for mm-scaled 3D measurements in a wide range of materials.

In this tutorial, the femtosecond laser enabled TriBeam microscope for use in tomography and bulk sample excavation is discussed, which will include the following topics:


Biological Sciences Tutorials

X43 - Liquid Cell TEM Imaging Techniques and Optimization for Biological Samples

Madeline Dukes, Protochips, Inc.

Madeline Dukes is an applications scientist with Protochips, Inc. She has more than 10 years of experience in the field of liquid-cell transmission electron microscopy (LC-TEM) and has worked with multiple labs around the world to improve in-situ LC-TEM technology and develop new sample preparation techniques for a broad range of applications.

X44 - Optimization of Cryo-EM Data Collection using Advanced Direct Detectors

Michael Spilman, Direct Electron

Michael is broadly interested in applying novel imaging methods using direct electron detectors to bridge high resolution biological structures with real-time dynamics. At Direct Electron, he supports and collaborates with customers on numerous projects including single particle cryoEM, microED, and liquid cell in situ.

X45 - CryoEM Sample Preparation: Problems and Potential Solutions

Alex Noble, New York Structural Biology Center

Alex is broadly interested in developing and applying methods for solving biomedical problems. He is the primary tomographer at SEMC. He works on several projects including cryo-FIB/SEM, tomography of cells, reconstituted systems, purified protein samples, tomography software development, and deep learning applications in cryoEM.


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