Physical Sciences Tutorials
X40 - Advanced Cryo-FIB Specimen Preparation and Handling of Environmentally Sensitive Materials for APT and TEM Analysis
Daniel Perea, Pacific Northwest National Laboratory
Dr. Perea is a senior staff scientist at the Environmental Molecular Sciences Laboratory at the Pacific Northwest National Laboratory. He received his Ph.D. in materials science & engineering from Northwestern University in 2009. Dr. Perea has over 14 years of experience using atom probe tomography, with much of his expertise in semiconductor and structural materials. His current interests at PNNL include developing unique cryogenic-based hardware and protocols to pioneer the use of atom probe tomography to probe the composition and structure of hydrated materials.
- Applications of APT to hydrated interfaces such as those in biological and environmental materials
- Development of a site-specific liftout and attachment scheme for cryogenically cooled specimens
- Challenges of handling and transfer of post cryo-prepared specimens between tools.
- Specimen shuttle suitcase devices for transfer of environmentally sensitive materials between the FIB and other tools enabling APT and TEM analysis
X41 Entrepreneurship in the Microscopy Community
Benjamin Bammes, Direct Electron
Benjamin has a broad scientific background, including physics, computer science, biology, and chemistry, working with small businesses related to science since 2003 in addition to academic research. He has a Ph.D. in Molecular Biophysics from Baylor College of Medicine, where he worked under National Academy of Sciences member Dr. Wah Chiu to optimize cryo-EM data collection and image processing. In 2011, Benjamin joined Direct Electron and now leads our collaborative approach to delivering high-tech solutions to the scientific community.
Several entrepreneurs from the microscopy community participate in an open Q&A forum on topics related to starting and running a microscopy-related business. Topics may include:
- How to identify and develop potential new products
- Steps for successful commercialization
- Methods for funding the early stages of a business
- Marketing and gaining initial customers
- Protecting and leveraging intellectual property
- The role of academic collaborations
Biological Sciences Tutorials
X43 - Liquid Cell TEM Imaging Techniques and Optimization for Biological Samples
Madeline Dukes, Protochips, Inc.
- Liquid Cell Transmission Electron Microscopy (LC-TEM) for biological systems — challenges & applications
- Strategies to minimize beam and radiolysis damage when imaging biological samples using LC-TEM
- Selecting the best parameters for imaging biological samples in liquid, including E-chip selection, flow conditions, etc.
- Sample preparation workflows for optimizing LC-TEM experiments, such as affinity-coating, staining, cell culture etc.
X44 - Optimization of Cryo-EM Data Collection using Advanced Direct Detectors
Michael Spilman, Direct Electron
- Optimizing CryoEM data collection for direct electron detectors
- When to use counting and linear modes for certain experiments
- Discussion of single particle, tomography, microED, and additional methods using direct electron detectors
X45 - CryoEM Sample Preparation: Problems and Potential Solutions
Alex Noble, New York Structural Biology Center
- What potential air-water interface issues exist in cryo-EM/ET?
- Why is preferred orientation an issue?
- How may air-water interface issues be reduced or avoided?
- How may preferred orientation issues be ameliorated?
- What are the basics for preparing cell samples for cryo-FIB/SEM?