Physical Sciences Tutorials

X41 - Entrepreneurship in the Microscopy Community

Several entrepreneurs from the microscopy community will be in attendance for a round table Q&A with tutorial attendees on topics including, but not limited to:

• Instrumentation development and commercialization
• Practical steps to take when starting your own microscopy based business
• Panel discussion on business start-up best practices
• Role of local affiliated microscopy societies in bringing microscopists and businesses together

X42 - Probing Ultralow Energy Excitations at Ultrahigh Spatial Resolution with Monochromated Electron Energy Loss Spectroscopy

PRESENTER:
Dr. Jordan A. Hachtel, Oak Ridge National Laboratory

• Introduction to monochromated EELS
• Alignment and tuning in a monochromated Nion UltraSTEM
• Different types of monochromated EELS experiments: Aloof EELS, off-axis EELS, momentum-resolved EELS
• Post-acquisition processing and analysis of ultralow-loss EELS data

Jordan Hachtel is a staff scientist at Oak Ridge National Laboratory. His research focuses on applications of ultralow-loss monochromated EELS to optical, biological, and quantum materials. Beyond this wide class of applications, he has also focused on using novel techniques, unique to ultralow-loss EELS, to access new aspects of the nanoscale infrared response in such systems.

 

X43 - X-Ray Imaging & Computed Tomography

PRESENTERS:
Tara Selly, Assistant Professor of Research, Univ. of Missouri
Jim Schiffbauer, Associate Professor, Geological Sciences, Univ. of Missouri

• Introduction to X-Ray microscopy and computed tomography
• How spot size, detector pixel size and geometrical magnification affect resolution
• Reconstruction strategies for isosurface determination
• Example applications in the geological sciences

 

Biological Sciences Tutorials

X44 - Cryo-EM Structure Determination of Small Proteins

PRESENTER:
Dr. Deb Kelly, Professor of Biomedical Engineering, Pennsylvania State University

• Isolating proteins from COVID-19 patients
• Affinity-capture using alternative substrates
• Low-dose Image Acquisition in Milli-seconds (LIAM)
• Reconstructing small proteins (~50 kDa range)

Dr. Kelly's research focuses on innovative approaches to study biological systems, ranging from human viruses to cancer. Central to their work is high-resolution imaging, primarily cryo-Electron Microscopy (EM). Dr. Kelly's team has developed a tunable microchip toolkit to study low-molecular weight proteins at high-resolution. Using these new tools, they expect to determine strategic insights for "Structural Oncology" applications.

X45 - Traversing Spatial Scales with Correlative Microscopy

PRESENTER:
James A.J. Fitzpatrick, Ph.D., Scientific Director, Center for Cellular Imaging, Professor of Neuroscience and Cell Biology & Physiology, Washington University School of Medicine.

• Introduction to Multi-Modal imaging and concept of Correlative Microscopy
• Discussion of current CLEM, CXREM and Cryo-CLEM methods
• Practical use of different algorithms for correlating multi-modal data
• Examples of Correlative Microscopy in Studies of Disease Pathogenesis

James Fitzpatrick is a Professor at Washington University School of Medicine in St. Louis and the inaugural Scientific Director of the Center for Cellular Imaging. His research focuses on the development and application of multi-modal correlated imaging approaches and AI-based image analysis methods to study the pathogenesis of cancer and neurodegenerative disease.