Technologists' Forum

X30 - Technologists' Forum Roundtable: Image Data Analysis Software Review

With the introduction of new 3D imaging microscope systems, data size and complexity has increased requiring more advanced software to analyze the results. Although many classic data analysis programs exist, there has been the development of new programs that are stand alone or integrated into microscope systems that have enhanced capabilities and improved user interfaces. This session will review a few new programs used to analyze data from whole slide scanners, light sheet imaging systems and 3D fluorescent image data.

• Analysis of whole slide scanner data using deep learning AI for multiplex data.
• Automated 3D analysis workflows for Light sheet, SEM, FIB-SEM, CLEM and other volume image data sets.
• 3D/4D visualization and analysis for cell-based segmentation, automated tracking and identification of intracellular relationships within 3D fluorescent image data sets.

ORGANIZERS:
D. Page Baluch, Arizona State University
Frank P. Macaluso, Albert Einstein College of Medicine

X31 - Technologists' Forum Symposia: 3D SEM Techniques

Interest in three-dimensional scanning electron microscopy has increased over the past few years with the expanded capability to combine Correlative Electron Microscopy (CLEM) with 3-D volume analysis. As more tools and workflows become available, the techniques in sample preparation and data analysis are still challenging. This year the Technologists' Forum is partnering with the organizers of the 3D Volume Electron Microscopy in Biological Science symposium to provide a series of presentations that focus on sample preparation, image acquisition and analysis.

• Review of the challenges and new developments in sample preparation for volume EM.
• Experimental design and workflows for sample preparation, image acquisition and analysis.
• Tips and tricks on 3-DSEM image acquisition and analysis.

ORGANIZERS:
D. Page Baluch, Arizona State University
Ru-Ching Hsia, Carnegie Institution for Science

X32 - Technologists' Forum Workshop — Tissue Clearing Tips & Techniques

With an increased interest in 3D tissue imaging, researchers have had to develop protocols to optically clear thick tissues for light microscopy-based applications. Tissue clearing is now combined with many other techniques such as multiplexing protein imaging, fluorescent in situ hybridization for DNA and RNA, super-resolution imaging techniques, and multiomics studies. This year the Technologists' Forum is partnering with the organizers of the Development, Challenges and Biomedical Applications of Tissue Clearing, Expansion Microscopy and Volumetric Imaging symposium to provide a series of presentations that will review the most common protocols used in tissue clearing, highlighting the challenges, and describing sample processing workflows.

• Identify the clearing technique most suited for your tissue type and imaging method.
• Learn about the most common clearing techniques used in light-based microscopy.
• Tips in avoiding tissue or objective lens/microscope system damage.

ORGANIZERS:
D. Page Baluch, Arizona State University
John L. Grazul, Cornell University
Ru-Ching Hsia, Carnegie Institution for Science