Microscopy Today Webinars
DSA Awardee Talk | From Deconvolution and Super-Resolution Light Microscopy to CryoEM Cameras to Beam Induced Motion Correction: Empowering Biology through Technology Developments
Date/Time: Thursday, November 18, 2021 | 1:00 PM EST
Date/Time: Thursday, November 18, 2021 | 1:00 PM EST Provided will be a brief summary of technological developments made by David Agard and John Sedat (UCSF) in pioneering 3D light and super-resolution microscopy, followed by a focus on cryoEM cameras culminating in the first single electron counting camera made in collaboration with Peter Denes at LBNL and Paul Mooney at Gatan. This will be followed by discussing our efforts in collaboration with Yifan Cheng (UCSF) to mitigate beam induced motion as a critical step in obtain high resolution reconstructions of biological systems.
Prof. David Agard | University of California, SF
A US biophysicist, David joined the faculty of the department of Biochemistry and Biophysics at the University of California, San Francisco in 1983. He is currently a Professor of Biochemistry & Biophysics and Professor of Pharmaceutical Chemistry. David was the founding Director of the California Institute for Bioengineering, Biotechnology and Quantitative Biomedical Research (QB3) in 2001, and was its UCSF Scientific Director from 2002-2006. He received his BS in Molecular Biophysics & Biochemistry from Yale in 1975 and PhD in Biological Chemistry from the California Institute of Technology in 1980. Having a strong background in structural biophysics, David’s current work focuses on elucidating the mechanisms of cytosolic protein homeostasis folding by the Hsp90-Hsp70 molecular chaperone system and the mechanisms by which giant bacteriophage evade host defenses by constructing multiple internal compartments. David has been instrumental in developing of direct phasing methods for SAXS, three dimensional deconvolution and Structured Illumination light microscopies, automated cryo-electron tomography, the K2 Summit single electron counting direct detector, and second-generation beam-induced motion correction software. His work has been recognized by his election to the National Academy of Sciences in 2007 and American Academy of Arts and Sciences in 2009. He currently serves on numerous scientific advisory boards.
DSA Awardee Talk | A Personal Journey to See Atoms in Matter
Date/Time: Thursday, November 4, 2021 | 1:00 PM EST
Atoms were introduced in antiquity as a philosophical term, then in the nineteenth century as a theoretical physical concept. However, no one believed that atoms could ever be seen. This changed during the twentieth century when people set out to develop microscopies that would allow them to associate a direct visual concept with the notion of atoms. I had the privilege of being involved in one of these developments. This is the evolution of transmission electron microscopy to atomic resolution. On the one hand, this involves the development of the associated electron optics. On the other hand, which is particularly important to me as a materials scientist, this concerns the first applications of atomic electron microscopy to problems in materials science. For this purpose, a very few, easy-to-see examples will be given, which are interesting from a general point of view and may contribute one or the other aspect to answering the question of what we can do now and possibly in the future. This is a very personal account of the journey toward seeing atoms in matter. This journey has changed the world around us, as well as ourselves. Therefore, when we emphasize the potential for application today, we should not lose sight of the worldview and aesthetic aspects of seeing atoms.
Prof. Knut Wolf Urban | Ernst Ruska Center, Research Center Juelich, Germany
Knut W. Urban studied physics at the University of Stuttgart where he received his doctorate degree in 1972. He then joined the Max Planck Institute for Metals Research, and he spent extended research stays at CEN Saclay/Paris, at Bhabha Center, Mumbai, and at Tohoku University, Sendai. In 1987 he became the Chair of Experimental Physics at RWTH Aachen University and the Director of Institute for Solid State Research, Research Center Juelich. In 2004 he became a co-founder of Ernst Ruska Center for Microscopy and Spectroscopy with Electrons. Since 2009 he has been a JARA Distinguished Professor. He worked in many fields of experimental solid-state physics. Currently, Prof. Urban’s main interest is in structural and physical properties of oxide materials. He was President of the German Physical Society. For the co-development, 1997, of the first aberration-corrected transmission electron microscope he received a number of awards. Among these are the US MRS von Hipel Award, and (with Haider and Rose) the HONDA Prize for Ecotechnology, the BBVA Award in Basic Sciences, the WOLF Prize in Physics and the KAVLI Prize for Nanoscience. He has an honorary degree of Tel Aviv University, and he is an honorary member of many national and international materials societies.
DSA Awardee Talk | The Path to the First CS-Corrected TEM
Date/Time: Thursday, October 21, 2021 | 1:00 PM EST
This webinar will describe how the development of the first aberration corrected TEM, with which the point resolution could significantly be improved, could be carried out and which troubles or difficulties had to be solved. The continuation of the development advanced electron optical systems from the first working high resolution EM till today will also be highlighted.
- Electron Optics
- Development of Aberration Corrector
- High Resolution EM
Prof. Dr. Maximilian Haider | CEOS GmbH
Maximilian Haider was born 1950 in Freistadt/Austria and after an apprenticeship as eye-optician he studied physics in Kiel and Darmstadt and received his PhD in 1987 in Darmstadt. For his PhD-thesis he developed an electron energy loss spectrometer for STEM which was carried out at the European Molecular Biology Laboratory (EMBL). In 1989 he became a Group Leader within the Physical Instrumentation Program at the EMBL. In 1992 he started a project to set up a Cs-corrector for a 200 kV TEM together with Prof. Rose in Darmstadt and Prof. Urban in Juelich. In 1996 he founded CEOS GmbH in Heidelberg together with Joachim Zach. In 1997 he successfully finished the Cs-Corrector project and since this time he received many outstanding awards – most of them together with H. Rose and K. Urban.
Ultrafast Transmission Electron Microscopy: Techniques and Applications
Date/Time: Tuesday, May 11, 2021 | 12:00 PM EST
With the growing applications of temporally-resolved electron microscopy for basic phenomena and reducing damage in samples, this webinar will provide an introduction to the field of ultrafast transmission electron microscopy, including techniques, equipment and historical perspectives from technical experts. A combination of historical developments with recent advances will help attendees in considering ultrafast capabilities for their own research, or satisfy general interests. The ultrafast technology review will also include a broader scope of applications enabled by ultrafast techniques using a variety of sample stimuli from multidisciplinary fields.
The objectives of this webinar:
- An appreciation of the growth of UTEM across many technical fields
- An understanding of the myths and legends of implementing UTEM
- A deep understanding of the methods available to achieve time-resolved EM
- A spark to research additional ultrafast applications
Dr. Eric Montgomery, Director of Research | Euclid Techlabs, LLC
I lead Euclid's Research Department: it's scientific team, synergistic project portfolio, and collaborative outreach. I have been awarded and led millions of dollars in research grant funding from the US DOE SBIR and STTR programs from 2012-present, particularly novel electron source development, advanced particle accelerator concepts, and novel electron microscopy technologies. My Ph.D. in Physics is from the University of Maryland College Park (2010), where I served as Assistant Research Scientist at the Institute for Research in Engineering and Applied Physics until 2018. I also enjoyed a four year part-time stint as an Adjunct Instructor at UMBC. While at Maryland I personally advised the research of over 30 university students. I have over 40 publications, two R&D 100 awards and three US patents.
Dr. John Roehling, Staff Scientist | Lawrence Livermore National Laboratory
My science career started at the University of California, Davis researching polymer photovoltaics, using transmission electron microscopy (TEM) and tomography to look at the structure of polymer/fullerene networks. Afterwards, I started at LLNL in 2014 working with pulsed TEM imaging examining the physics of solidification in metal alloys. This gained me ample experience with laser systems and solidification theory. Most recently I have branched out into laser-based additive manufacturing and have been working toward using pulsed electron imaging to examine the rapid solidification that occurs in additive manufacturing in finer detail.
Utilizing Dynamic CT Imaging for True In Situ Experimentation
Thursday, August 13, 2020 | 1:00 p.m. ET
The technique of time-resolved 3D imaging with X-rays has rapidly emerged as an essential part of a researcher's toolkit for understanding materials evolution. The expansion into "4D" has facilitated in situ investigations ranging from mechanical deformation of solids to fluid flow in porous materials to the evolution of structure in changing environmental conditions. Imaging of dynamic processes specifically processes happening over a short time-period, has been one of the key applications at synchrotron micro-CT beamlines, with temporal resolutions well below one second. However, access to these facilities is often limited, with respect to both time at the beamline and application specificity.
In the laboratory, image quality and spatial resolution have been significantly improved over the last decade, providing researchers routine and regular access to very high quality computed tomography data. However, the advances in temporal resolution have been less accelerated, especially for imaging dynamic processes. Lab-based CT has been limited to pseudo in situ experimentation where data are collected on interrupted processes allowing for the traditionally longer and non-continuous scanning protocols. Additionally, analyzing and visualizing complicated data from 3D studies has been fairly burdensome. Over the last several years, development at TESCAN has focused on addressing these problems and has now made it possible to acquire, visualize, and analyze micro-CT data on uninterrupted and dynamic processes. Through a number of hardware and software advances, temporal resolution has been pushed below 10 seconds per scan, opening up new frontiers for lab-based 4D CT work.
In this talk we explore the challenges and innovations that have led to dynamic CT, highlighting several applications across materials sciences, life sciences, and geosciences. Click here to register.
Luke Hunter is the regional product manager for North and South America for TESCAN’s Micro-CT product lines. He has over a decade of experience in the X-ray microscopy and micro-CT industries, as an application specialist, applications manager, and product manager. After extensive time focusing on high resolution computed tomography, Luke was very excited by the opportunity to join TESCAN and push the boundaries of the field through lab-based dynamic CT. He received his master’s degree from the University of California, Berkeley (Berkeley, CA USA) in Mechanical Engineering and his bachelor’s degree, also in Mechanical Engineering, from Washington State University (Pullman, WA USA).
TESCAN is a global supplier of scientific instrumentation and microscopy solutions, headquartered in Brno, Czech Republic. The company is focused on research, development, and manufacturing of scientific instruments and laboratory equipment including electron- and ion microscopy, micro-CT, and more.